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Entry M0277    1.16.1.1    mercury(II) reductase

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Step 14

Cys136 abstracts a proton from the flavin-mercury adduct, initiating a reductive elimination of the mercury metal and thiolate Cys141.

GIF of Reaction Step M0277.stg14



Mechanisms

Proton Transfer
Elimination Reaction

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Cofactor Regenerated
Overall Product Formed
Enzyme-Substrate Bond Cleavage

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain spectator Not Active
Met9A Side Chain spectator Steric Role
Tyr65A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Cys559B Side Chain spectator Not Active
Cys14A Side Chain spectator Not Active
Cys11A Side Chain spectator Not Active
Cys141A Side Chain reactant Activator
Nucleofuge
Cys136A Side Chain reactant Activator
Proton Acceptor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A reactant Activator
Proton Donor
Electrofuge

Metal Cofactors involved in Step 14

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB reactant Two Electron Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-H
N-H
The C-N bond changes from a single to double bond
C
H
N
S

View similar reactions in MACiE.


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