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Entry M0277    1.16.1.1    mercury(II) reductase

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Step 11

Cys141 displaces Cys558' within the metal coordination sphere.

GIF of Reaction Step M0277.stg11



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Formation
Bond Cleavage
Metal Coordination
Metal De-coordination
Enzyme-Substrate Bond Formation
Enzyme-Substrate Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain reactant Activator
Electrofuge
Met9A Side Chain spectator Steric Role
Tyr65A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Cys559B Side Chain spectator Not Active
Cys14A Side Chain spectator Not Active
Cys11A Side Chain spectator Not Active
Cys141A Side Chain reactant Activator
Nucleophile
Cys136A Side Chain spectator Covalently Attached

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A spectator Electrostatic Stabiliser
Increase Acidity

Metal Cofactors involved in Step 11

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Hg-S
Hg-S
None
Hg
S

View similar reactions in MACiE.


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