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Entry M0277    1.16.1.1    mercury(II) reductase

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Step 09

Cys136 acts as a nucleophile towards the close proximity mercury-thiol adduct, forming another metal-ligand bridging intermediate.

GIF of Reaction Step M0277.stg09



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Intermediate Formation
Enzyme-Substrate Bond Formation
Enzyme-Substrate Bond Cleavage

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain reactant Activator
Metal Ligand
Electrostatic Stabiliser
Met9A Side Chain spectator Steric Role
Increase Nucleophilicity
Tyr65A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Cys559B Side Chain reactant Activator
Nucleofuge
Cys14A Side Chain spectator Not Active
Cys11A Side Chain spectator Not Active
Cys141A Side Chain spectator Not Active
Cys136A Side Chain reactant Activator
Nucleophile

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A spectator Electrostatic Stabiliser
Increase Acidity

Metal Cofactors involved in Step 09

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Hg-S
Hg-S
None
Hg
S

View similar reactions in MACiE.


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