Entry M0277    mercury(II) reductase

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Step 08

The thiolate form of Cys14 abstracts a proton from the positively charged sulfur of Cys558'.

GIF of Reaction Step M0277.stg08


Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain reactant Activator
Metal Ligand
Proton Donor
Met9A Side Chain spectator Steric Role
Electrostatic Stabiliser
Tyr65A Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Cys559B Side Chain reactant Activator
Covalently Attached
Electrostatic Stabiliser
Cys14A Side Chain reactant Activator
Proton Acceptor
Cys11A Side Chain spectator Electrostatic Stabiliser
Cys141A Side Chain spectator Not Active
Cys136A Side Chain spectator Hydrogen Bond Acceptor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A spectator Electrostatic Stabiliser
Increase Acidity

Metal Cofactors involved in Step 08

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved

View similar reactions in MACiE.