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Entry M0277    1.16.1.1    mercury(II) reductase

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Step 05

Cys559' acts as a nucleophile towards the Hg(II) metal, displacing it from the metal binding motif and relocating it to within the enzyme active site.

GIF of Reaction Step M0277.stg05


Comment: The mercury metal is transferred from the N terminal binding site to a thiol pair situated more closely within the active site [1].



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Enzyme-Substrate Bond Cleavage
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain spectator Not Active
Met9A Side Chain spectator Steric Role
Electrostatic Stabiliser
Tyr65A Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Cys559B Side Chain reactant Nucleophile
Metal Ligand
Cys14A Side Chain reactant Activator
Covalently Attached
Cys11A Side Chain reactant Activator
Covalently Attached
Nucleofuge
Metal Ligand
Cys141A Side Chain spectator Not Active
Cys136A Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A spectator Electrostatic Stabiliser
Increase Acidity

Metal Cofactors involved in Step 05

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Hg-S
Hg-S
None
Hg
S

View similar reactions in MACiE.


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