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Entry M0277    1.16.1.1    mercury(II) reductase

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Step 02

The thiolate anion formed on the substrate acts as a base towards the protonated Cys11.

GIF of Reaction Step M0277.stg02



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys558B Side Chain spectator Not Active
Met9A Side Chain spectator Steric Role
Electrostatic Stabiliser
Tyr65A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Cys559B Side Chain spectator Not Active
Cys14A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Cys11A Side Chain reactant Activator
Covalently Attached
Proton Donor
Metal Ligand
Cys141A Side Chain spectator Not Active
Cys136A Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD666A spectator Electrostatic Stabiliser
Increase Acidity

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
mercury No Information available from the PDB spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-H
S-H
None
H
S

View similar reactions in MACiE.


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