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Entry M0276    1.7.99.4    nitrate reductase

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Step 06

A solvent molecule acts as a proton donor, forming a water ligand within the metal coordination sphere.

GIF of Reaction Step M0276.stg06


Comment: The proton donor is presumed to be a solvent molecule because of the identification of solvent channels close to the metal coordination site. However, the possibility of a residue acting as a general acid has not been ruled out [1].



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Overall Reactant Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Met153A Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction
Steric Role
Lys56A Side Chain spectator Hydrogen Bond Donor
Cys152 Side Chain reactant Activator
Electrostatic Stabiliser
Covalently Attached
Gly383A Main Chain Amide spectator Hydrogen Bond Donor
Met346A Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction
Steric Role

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Molybdopterin guanosine dinucleotide MGD1803A spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Metal Ligand
Molybdopterin guanosine dinucleotide MGD1804A spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Metal Ligand

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
iron SF4 1801 A spectator Not Active
molybdenum MO 1802 A spectator Not Active
iron HEC 1128 B spectator Not Active
iron HEC 1129 B spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
None
H
O

View similar reactions in MACiE.


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