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Entry M0276    1.7.99.4    nitrate reductase

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Step 03

The close proximity iron sulfur cluster provides an electron to the molybdenum centre via a bridging lysine residue.

GIF of Reaction Step M0276.stg03


Comment: The electron is relayed to the iron-sulfur cluster from a mobile electron carrier present in the quinol oxidation respiratory electron transport chain [4].



Mechanisms

Electron Transfer

Mechanism Components

Cofactor Used
Cofactor Regenerated
Intermediate Formation
Electron Relay
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Met153A Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction
Steric Role
Lys56A Side Chain reactant Activator
Electron Relay
Hydrogen Bond Donor
One Electron Acceptor
One Electron Donor
Cys152 Side Chain reactant Activator
Metal Ligand
Covalently Attached
Gly383A Main Chain Amide spectator Hydrogen Bond Donor
Met346A Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction
Steric Role
Glu384A Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Molybdopterin guanosine dinucleotide MGD1803A spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Metal Ligand
Molybdopterin guanosine dinucleotide MGD1804A spectator Electrostatic Stabiliser
Metal Ligand

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
iron SF4 1801 A reactant Electron Relay
One Electron Acceptor
One Electron Donor
molybdenum MO 1802 A reactant One Electron Acceptor
iron HEC 1128 B reactant Electron Relay
One Electron Acceptor
One Electron Donor
iron HEC 1129 B reactant Electron Relay
One Electron Acceptor
One Electron Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
None
None

View similar reactions in MACiE.


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