Entry M0274 18.104.22.168 pyruvate oxidase
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Phosphate initiates a nucleophilic attack on the kinetically stable anion radical adduct.
Comment: Phosphate attacks the kinetically stable anion radical adduct. Kinetics experiments have shown the presence of phosphate to enhance the rate of electron transfer . The negative charge resulting from the nucleophilic attack by phosphate is thought to reduce the potential of the intermediate and therefore increase the driving force of the following electron transfer to FAD.
An alternative mechanism has been proposed in  which involves the homolytic fragmentation of a phosphate radical adduct.
Amino acids involved in the reaction step.
Organic Cofactors involved in the reaction step
Metal Cofactors involved in Step 07
||Bonds Changed in Order
||Atom Types Involved
The C-C bond changes from a double to single bond
View similar reactions in MACiE.