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Entry M0272    4.1.3.1    isocitrate lyase

Next Step

Step 01

His193 deprotonates Cys191 which in turn deprotonates the alpha carbon of succinate to form the enolte.

GIF of Reaction Step M0272.stg01


Comment: It has been suggested that Cys191 is the most appropriate residue to abstract the alpha-proton from the succinate substrate [1].



Mechanisms

Proton Transfer
Assisted Keto-Enol Tautomerisation

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Proton Relay
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His193 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Cys191 Side Chain reactant Hydrogen Bond Donor
Hydrogen Bond Acceptor
Proton Acceptor
Proton Donor
Proton Relay
Arg228 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His180 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser315 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser317 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG 451 A spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
S-H
C-H
S-H
The C-C bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O
S

View similar reactions in MACiE.


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