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Entry M0271    5.4.2.9    phosphoenolpyruvate mutase

Previous Step

Step 02

The oxyanion initiated a nulceophilic attack of the C3 on the metaphosphate to form the product.

GIF of Reaction Step M0271.stg02


Comment: The metaphosphate's interactions hole is stationary, whilst the C1-C2 bond os the pyruvate enolate rotates so that the C3-P bond can be formed [2].



Mechanisms

Bimolecular Nucleophilic Addition

Mechanism Components

Bond Formation
Bond Order Change
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg159 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser46 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Steric Role
Leu48 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Steric Role
Ser123 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asn122 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His190 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium MG 1003 A spectator Electrostatic Stabiliser
Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-P
None
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
C
O
P

View similar reactions in MACiE.


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