Overview for MACiE Entry M0271
EC Number: 126.96.36.199 (A member of the Isomerases, Intramolecular transferases, Phosphotransferases (phosphomutases))
Enzyme Name: phosphoenolpyruvate mutase
Biological Species: Mytilus edulis (Blue mussel)
Catalytic Chain UniprotKB Accession Codes:
- P56839 - Phosphoenolpyruvate phosphomutase
Representative PDB Code: 1pym - PHOSPHOENOLPYRUVATE MUTASE FROM MOLLUSK IN WITH BOUND MG2-OXALATE (Resolution = 1.80 Å).
Catalytic CATH Codes:
Display structure information
Overall Comment: There are three alternative mechanisms by which a phosphoryl transfer may proceed: (i) dissociative with a trigonal metaphosphate intermediate, (ii) concerted, associative transfer, involving a trigonal bipyramidal transition state, (iii) stepwise associative transfer (the addition-elimination pathway), involving a trigonal bipyramidal intermediate. The initial proposal had Asp58 acting as the phosphoryl carrier . However, subsequent studies have suggested that the dissociative mechanism (whilst unusual) is more likely for this enzyme [2,3,4]. However, it is still unclear whether the reaction proceeds via a distinct metaphosphate intermediate, or a highly separated transition state .
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Stepwise Description of the Reaction
|Step 1||The metaphosphate dissociates from the phosphoenolpyruvate substrate.|
|Step 2||The oxyanion initiated a nulceophilic attack of the C3 on the metaphosphate to form the product.|
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Catalytic Residues Involved
||Location of Function
||Main Chain Amide
Metal Cofactors for M0271
- K. Huang et al. (1999), Structure Fold Des., 7, 539-548. Helix swapping between two alpha/beta barrels: crystal structure of phosphoenolpyruvate mutase with bound Mg(2+)-oxalate.
- S. Liu et al. (2002), Biochemistry, 41, 10270-10276. Dissociative Phosphoryl Transfer in PEP Mutase Catalysis: Structure of the Enzyme/Sulfopyruvate Complex and Kinetic Properties of Mutants.
- 3) D. Xu et al. (2008), J. Phys. Chem. B, 112, 4102-4108. Ab initio QM/MM studies of the phosphoryl transfer reaction catalyzed by PEP mutase suggest a dissociative metaphosphate transition state.
- D. Xu et al. (2005), J. Phys. Chem. B, 109, 13827-13834. Theoretical studies of dissociative phosphoryl transfer in interconversion of phosphoenolpyruvate to phosphonopyruvate: solvent effects, thio effects, and implications for enzymatic reactions.
Homologue information for M0271 (1pym)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Entries with at least one Catalytic CATH code in common (different mechanisms):
|M0326 ||pyruvate kinase |
|M0207 ||pyruvate, phosphate dikinase |
|M0272 ||isocitrate lyase |
|M0182 ||methylisocitrate lyase |
View a comparison of the other reactions in MACiE with the CATH domain 188.8.131.52
Links to this entry in other databases