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Entry M0269    5.5.1.1    muconate cycloisomerase

Previous Step

Step 03

In an inferred return step, Lys169 deprotonates water to regenerate the active site.

GIF of Reaction Step M0269.stg03


Comment: Inferred return step. Alternatively, the enzyme may catalyse the reverse reaction, in which the Lys169 deprotonates the alpha carbon of 2,5-dihydro-5-oxofuran-2-acetate.



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu327 Side Chain spectator Hydrogen Bond Acceptor
Lys169 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Lys167 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
manganese MN 374 A spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
None
H
N
O

View similar reactions in MACiE.


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