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Entry M0263    4.2.3.37    epi-isozizaene synthase

Next Step

Step 01

The binding of the substrate causes a conformation change in the active site, which promotes the elimination of the pyrophosphate.

GIF of Reaction Step M0263.stg01



Mechanisms

Intramolecular Elimination

Mechanism Components

Bond Cleavage
Bond Order Change
Charge Delocalisation
Dephosphorylation
Intermediate Formation
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg338 Side Chain spectator Electrostatic Stabiliser
Promotes Heterolytic Reactions
Hydrogen Bond Donor
Phe95 Side Chain spectator Steric Role
Electrostatic Stabiliser
Polar Non-polar interaction
Phe96 Side Chain spectator Steric Role
Electrostatic Stabiliser
Polar Non-polar interaction
Phe198 Side Chain spectator Steric Role
Electrostatic Stabiliser
Polar Non-polar interaction

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG 700 A spectator Substrate Binding
Activator
Promotes Heterolytic Reactions
Electrostatic Stabiliser
magnesium MG 701 A spectator Substrate Binding
Activator
Promotes Heterolytic Reactions
Electrostatic Stabiliser
magnesium MG 702 A spectator Substrate Binding
Activator
Promotes Heterolytic Reactions
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
C-O
The C-C bond changes from a single to double bond
The C-C bond changes from a double to single bond
C
O

View similar reactions in MACiE.


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