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Overview for MACiE Entry M0255

Version history

General Information

EC Number: 1.1.1.1 (A member of the Oxidoreductases, Acting on the CH-OH group of donors, With NAD+ or NADP+ as acceptor)

Enzyme Name: alcohol dehydrogenase

Biological Species: Drosophila melanogaster (Fruit Fly)

Catalytic Chain UniprotKB Accession Codes:

  • P00334 - Alcohol dehydrogenase

Representative PDB Code: 1mg5 - CRYSTAL STRUCTURE OF DROSOPHILA MELANOGASTER ALCOHOLDEHYDROGENASE COMPLEXED WITH NADH AND ACETATE AT 1.6 A (Resolution = 1.63 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of  alcohol

Image of NAD

right arrow

Image of aldehyde or ketone

Image of proton

Image of NADH

alcohol
C00069
CHEBI:30879
NAD
C00003
CHEBI:15846
aldehyde or ketone
C01450
CHEBI:23019
proton
C00080
CHEBI:15378
CHEBI:24636
NADH
C00004
CHEBI:16908

Overall Comment: This alcohol dehydrogenase is zinc-independent and is a member of the SDR family.


View similar reactions


Stepwise Description of the Reaction

Step 1Bulk water initiates the deprotonation of Tyr152 via Lys156, the ribose ring of NAD850. Tyr152 deprotonates the alcohol group, initiating hydride transfer to NAD850.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Tyr 152 A Side Chain
Lys 156 A Side Chain
Ser 139 A Side Chain

Organic Cofactors for M0255

Type Identity Chain
NAD NAD 850 A Overview

References

  1. J. Benach et al. (1999), J. Mol. Biol., 289, 335-355. The catalytic reaction and inhibition mechanism of Drosophila alcohol dehydrogenase: observation of an enzyme-bound NAD-ketone adduct at 1.4 A resolution by X-ray crystallography.
    Medline: 10366509
  2. R. Ladenstein et al. (2008), Cell. Mol. Life Sci., 65, 3918-3935. Structure-function relationships in short-chain alcohol dehydrogenases.
    Medline: 19011748

Homologue information for M0255 (1mg5)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with the same EC number (different mechanisms):

MACiE Entry Enzyme Name PDB code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0256 alcohol dehydrogenase 1qlh 0.931 0.225 Compare

Compare of the other reactions in MACiE with EC number 1.1.1.1


Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0092 UDP-glucose 6-dehydrogenase
1.1.1.22
1dli 3.40.50.720
0.50.1125Compare
M0021 malate dehydrogenase (oxaloacetate-decarboxylating)
1.1.1.38
1do8 3.40.50.720
0.56750.2999Compare
M0227 GDP-L-fucose synthase
1.1.1.271
1e6u 3.40.50.720
0.24030.5142Compare
M0110 D-amino-acid oxidase
1.4.3.3
1c0p 3.40.50.720
0.42370.18Compare
M0237 pteridine reductase
1.5.1.33
2c7v 3.40.50.720
0.55170.2229Compare
M0116 NAD(P)+ transhydrogenase (AB-specific)
1.6.1.2
1hzz 3.40.50.720
0.20.142Compare
M0142 ferredoxin-NADP+ reductase
1.18.1.2
1e6e 3.40.50.720
0.20510.0209Compare
M0090 adenosylhomocysteinase
3.3.1.1
1b3r 3.40.50.720
0.41530.1509Compare
M0226 UDP-sulfoquinovose synthase
3.13.1.1
1qrr 3.40.50.720
0.39340.5888Compare
M0228 dTDP-glucose 4,6-dehydratase
4.2.1.46
1bxk 3.40.50.720
0.37140.3589Compare
M0188 UDP-glucose 4-epimerase
5.1.3.2
1xel 3.40.50.720
0.53331Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.40.50.720


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
alcohol dehydrogenase (NAD) activity (molecular function)
binding (molecular function)
alcohol metabolic process (biological process)
metabolic process (biological process)
oxidoreductase activity (molecular function)
oxidation-reduction process (biological process)
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