Overview for MACiE Entry M0250
EC Number: 188.8.131.52 (A member of the Oxidoreductases, Acting on a peroxide as acceptor, Peroxidases)
Enzyme Name: chloride peroxidase
Biological Species: Caldariomyces fumago (Fungus)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 2cpo - CHLOROPEROXIDASE (Resolution = 2.10 Å).
Display structure information
Overall Comment: This is the heme-dependent chloroperoxidase.
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Stepwise Description of the Reaction
|Step 1||The hydrogen peroxide, once bound to the heme cofactor, undergoes self de-protonation to produce the activated species.|
|Step 2||The activated hydrogen peroxide species collapses, with concomitant movement of an electron pair to the iron centre of heme, and shuttling of a single electron out into the porphyrin ring of the cofactor. This generates the so-called compound I.|
|Step 3||The chloride ion initiates a nucleophilic attack on the oxo group of Compound I in an addition reaction which results in the donation of two electrons to the iron centre, and a single electron being transferred to the prophyrin ring.|
|Step 4||The intermediate deprotonates Glu183, which in turn deprotonates His105.|
|Step 5||His105 deprotonates Glu183, which in turn deprotonates the alkane. This initiates a nucleophilic attack upon the heme-bound hypochlorous acid in a substitution reaction.|
|Step 6||The bound hydroxide deprotonates a water molecule in an inferred return step.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0250
- W.-D. Woggon et al. (2001), J. Inorg. Biochem., 83, 289-300. Synthetic active site analogues of heme-thiolate proteins. Characterization and identification of intermediates of the catalytic cycles of cytochrome P450cam and chloroperoxidase.
- H.-A. Wagenknecht et al. (1997), Chem. Biol., 4, 367-372. Identification of intermediates in the catalytic cycle of chloroperoxidase.
- M. Sundaramoorthy et al. (1998), Chem. Biol., 5, 461-473. Stereochemistry of the chloroperoxidase active site: crystallographic and molecular-modeling studies.
Homologue information for M0250 (2cpo)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Entries with the same EC number (different mechanisms):
View a comparison of the other reactions in MACiE with EC number 184.108.40.206
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