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Entry M0237    1.5.1.33    pteridine reductase

Next Step

Step 01

NADPH transfers a hydride to the C7 position of biopterin. The N8 position is protonated by Tyr174, which in turn is protonated by Asp161. Since Asp161 is exposed to the bulk solvent it is then protonated by water.

GIF of Reaction Step M0237.stg01


Comment: Asp161 is exposed to the solvent.



Mechanisms

Hydride Transfer
Proton Transfer
Bimolecular Nucleophilic Addition
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Formation
Overall Product Formed
Proton Relay
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg14 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp161 Side Chain reactant Proton Relay
Proton Acceptor
Proton Donor
Hydrogen Bond Donor
Hydrogen Bond Acceptor
Tyr174 Side Chain reactant Proton Relay
Proton Acceptor
Proton Donor
Hydrogen Bond Donor
Hydrogen Bond Acceptor
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
N-H
O-H
O-H
C-H
O-H
O-H
O-H
The C-C bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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