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Entry M0234    6.3.5.2    GMP synthase (glutamine-hydrolysing)

Previous Step

Step 06

The tetrahedral intermediate collapses, liberating Cys86, which deprotonates His181, and the glutamate product.

GIF of Reaction Step M0234.stg06


Comment: Reaction occurs in the glutaminase domain (CATH 3.40.50.880).



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Enzyme-Substrate Bond Cleavage
Intermediate Terminated
Intermediate Collapse
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Gly59 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys86 Side Chain reactant Covalently Attached
Nucleofuge
Hydrogen Bond Acceptor
Proton Acceptor
His181 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Glu183 Side Chain spectator Hydrogen Bond Acceptor
Increase Acidity
Tyr87 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
magnesium MG 530 B spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-H
C-S
N-H
The C-O bond changes from a single to double bond
C
H
N
O
S

View similar reactions in MACiE.


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