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Entry M0229    6.3.2.1    pantoate-beta-alanine ligase

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Step 03

Beta-alanine initiates a nucleophilic attack on the carbonyl of the pantoyl adenylate intermediate to form a tetrahedral intermediate.

GIF of Reaction Step M0229.stg03



Mechanisms

Bimolecular Nucleophilic Addition

Mechanism Components

Bond Formation
Bond Order Change
Overall Reactant Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser196 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg198 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His44 Side Chain spectator van der Waals interaction
Hydrogen Bond Donor
Electrostatic Stabiliser
Ser197 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His47 Side Chain spectator van der Waals interaction
Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
magnesium MG 1001 _ spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-N
None
The C-O bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


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