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Entry M0223    6.4.1.1    pyruvate carboxylase

Previous Step

Step 07

Reaction occurs in the CT domain. Lys718 deprotonates the activated biotin, which in turn deprotonates Asp549, returning the enzyme to its ground state.

GIF of Reaction Step M0223.stg07


Comment: This step occurs in the Carboxyl Transferase (CT) domain.



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Cofactor Regenerated
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys718 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Asp549 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Asp655 Side Chain spectator Metal Ligand

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN reactant Hydrogen Bond Donor
Proton Donor
Hydrogen Bond Acceptor
Proton Acceptor

Metal Cofactors involved in Step 07

Metal Type Metal Identity Chain Activity Function
zinc ZN 1157 A spectator Substrate Binding
magnesium MG 1156 B spectator Not Present
magnesium MG 1157 B spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
N-H
O-H
O-H
The C-O bond changes from a single to double bond
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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