spacer

Entry M0223    6.4.1.1    pyruvate carboxylase

Previous Step     Next Step

Step 05

Reaction occurs in the CT domain. The Asp549 deprotonates pyruvate, activating the pyruvate in a keto-enol tautomerisation.

GIF of Reaction Step M0223.stg05


Comment: This step occurs in the Carboxyl Transferase (CT) domain.



Mechanisms

Proton Transfer
Assisted Keto-Enol Tautomerisation

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Formation
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys718 Side Chain spectator Hydrogen Bond Donor
Asp549 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Asp655 Side Chain spectator Metal Ligand
Activator

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN spectator Hydrogen Bond Acceptor

Metal Cofactors involved in Step 05

Metal Type Metal Identity Chain Activity Function
zinc ZN 1157 A spectator Substrate Binding
Activator
Increase Acidity
magnesium MG 1156 B spectator Not Present
magnesium MG 1157 B spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-H
The C-C bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


spacer
spacer