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Entry M0223    6.4.1.1    pyruvate carboxylase

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Step 03

Reaction occurs in the BC domain. The phosphate deprotonates one of the N-H groups of biotin with concomitant tautomerisation to produce an oxyanion.

GIF of Reaction Step M0223.stg03


Comment: This step occurs in the biotin carbozylase (BC) domain.



Mechanisms

Proton Transfer
Assisted Other Tautomerisation

Mechanism Components

Cofactor Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

There are no amino acid residues associated with this reaction step


Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN reactant Hydrogen Bond Donor
Proton Donor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
zinc ZN 1157 A spectator Not Present
magnesium MG 1156 B spectator Substrate Binding
magnesium MG 1157 B spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
N-H
The C-N bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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