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Entry M0223    6.4.1.1    pyruvate carboxylase

Next Step

Step 01

Reaction occurs in the BC domain. The bicarbonate is deprotonated by an unidentified base. The activate bicarbonate then acts as a nucleophile and attacks the gamma-phosphate in a substitution reaction, liberating ADP. Mg(II) stabilises/activates the ATP

GIF of Reaction Step M0223.stg01


Comment: This step occurs in the biotin carbozylase (BC) domain.



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

There are no amino acid residues associated with this reaction step


Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN spectator Not Active

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
zinc ZN 1157 A spectator Not Present
magnesium MG 1156 B spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity
magnesium MG 1157 B spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
R-H
O-H
P-O
The C-O bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
O
P
R

View similar reactions in MACiE.


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