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Entry M0222    4.1.2.13    fructose-bisphosphate aldolase (Class I)

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Step 03

Lys229 initiates an elimination of water, which gains an extra proton from Glu187.

GIF of Reaction Step M0222.stg03


Comment: Final step of Schiff base formation.



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Cleavage
Intermediate Formation
Intermediate Collapse
Enzyme-Substrate Bond Cleavage
Schiff Base Formed
Dehydration

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys229 Side Chain reactant Covalently Attached
Nucleophile
Hydrogen Bond Donor
Two Electron Donor
Glu187 Side Chain reactant Electrostatic Stabiliser
Hydrogen Bond Donor
Proton Donor
Polar Interaction
Glu189 Side Chain spectator Polar Interaction
Hydrogen Bond Acceptor
Electrostatic Stabiliser
Lys146 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser300 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Electrostatic Stabiliser
Asp33 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Tyr363 Side Chain spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-O
O-H
The C-N bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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