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Entry M0218    3.1.1.3    triacylglycerol lipase

Next Step

Step 01

His263 (part of a Ser-His-Asp triad) deprotonates Ser152, which attacks the carboxy carbon of the triacylglycerol substrate in a nucleophilic addition.

GIF of Reaction Step M0218.stg01


Comment: Serine attacks on the Re face [3]



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Overall Reactant Used
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Leu153 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser152 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Nucleophile
Phe77 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His263 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Acceptor
Asp176 Side Chain spectator Activator
Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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