EC Number: 126.96.36.199 (A member of the Lyases, Carbon-carbon lyases, Aldehyde-lyases)
Enzyme Name: hydroxynitrilase
Biological Species: Hevea brasiliensis (Para rubber tree)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 1sc9 - HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS IN COMPLEX WITH THENATURAL SUBSTRATE ACETONE CYANOHYDRIN (Resolution = 1.80 Å).
Overall Comment: There is no evidence that the mechanism of the H. brasiliensis enzyme differs from that of the Manihot esculenta enzyme, although their substrate specificity is slightly different. The enzyme from H. brasiliensis tolerates aliphatic (including alpha,beta-unsaturated), aromatic and heterocyclic aldehydes, whereas the enzyme from M. esculenta catalyses the addition of hydrogen cyanide to aliphatic, aromatic, heteroaromatic aldehydes and methyl ketones. Both are specific to the S enantiomer. The overall reaction catalysed is very reversible and of importance to synthetic organic chemists.
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Stepwise Description of the Reaction
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Catalytic Residues Involved
Homologue information for M0217 (1sc9)
Entries with at least one Catalytic CATH code in common (different mechanisms):
View a comparison of the other reactions in MACiE with the CATH domain 188.8.131.520
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