spacer

Entry M0206    5.4.2.6    beta-phosphoglucomutase

Previous Step     Next Step

Step 03

The bisphosphate intermediate rotates in the active site. Asp1008 attacks the C1 phosphate of the intermediate in a nucleophilic addition. This produces a pentavalent, covalently attached intermediate.

GIF of Reaction Step M0206.stg03


Comment: The beta-D-glucose bisphosphate rotates within the active site before this step can occur. It is assumed that this step also proceeds via a pentavalent phosphate intermediate.



Mechanisms

Bimolecular Nucleophilic Addition

Mechanism Components

Bond Formation
Bond Order Change
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser1114 Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Asp1008 Post-Translationally Modified Residue reactant Nucleophile
Polar Interaction
Ala1115 Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Val1009 Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Lys1145 Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Asp1010A Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Asp1010A Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
magnesium MG 2800 _ spectator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
None
The P-O bond changes from a double to single bond
O
P

View similar reactions in MACiE.


spacer
spacer