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Entry M0201    6.4.1.3    propionyl-CoA carboxylase

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Step 06

Reaction occurs in the beta-subunit. The oxyanion re-forms the carbonyl group, causing the C=N bond of the activated biotin to deprotonate the propanoyl-CoA with concomitant tautomerisation. The main chain amides of Gly182 and Gly183 stabilise the activated propanoyl-CoA, the main chain amides of Gly419B and Ala420B stabilise the oxyanion of the activated biotin.

GIF of Reaction Step M0201.stg06


Comment: This step occurs in the beta-subunit, the carboxytransferase domain.



Mechanisms

Proton Transfer
Assisted Other Tautomerisation
Assisted Keto-Enol Tautomerisation

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Intermediate Terminated
Cofactor Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Gly182 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly183 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly419B Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ala420B Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN5600 reactant Hydrogen Bond Acceptor
Proton Acceptor

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-H
The C-C bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-N bond changes from a double to single bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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