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Entry M0201    6.4.1.3    propionyl-CoA carboxylase

Next Step

Step 01

Reaction occurs in the alpha-subunit. The bicarbonate is deprotonated by an unidentified base. The activate bicarbonate then acts as a nucleophile and attacks the gamma-phosphate in a substitution reaction, liberating ADP. Mg(II) stabilises/activates the ATP

GIF of Reaction Step M0201.stg01


Comment: This step occurs in the alpha-subunit, the biotin carboxylase domain.



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

There are no amino acid residues associated with this reaction step


Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Biotin BTN5600 spectator Hydrogen Bond Donor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Substrate Binding
Activator
Increase Electrophilicity
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
R-H
O-H
P-O
The C-O bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
O
P
R

View similar reactions in MACiE.


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