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Entry M0200    6.3.1.5    NAD+ synthase

Previous Step

Step 03

Water deprotonates the ammonia molecule, which then acts as a nucleophile and attacks the carboxyl carbon of the phosphorylated intermediate in a substitution reaction, liberating AMP. A second water molecule deprotonates the alcohol group formed in the previous step, regenerating the carbonyl group.

GIF of Reaction Step M0200.stg03


Comment: It is possible that these two protonation events may occur in a stepwise, rather than concerted, manner.



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

There are no amino acid residues associated with this reaction step


Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
magnesium MG 5001 x spectator Substrate Binding
Electrostatic Stabiliser
magnesium MG 5002 x spectator Substrate Binding
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-N
O-H
O-H
C-O
N-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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