Entry M0200 22.214.171.124 NAD+ synthase
Water deprotonates the ammonia molecule, which then acts as a nucleophile and attacks the carboxyl carbon of the phosphorylated intermediate in a substitution reaction, liberating AMP. A second water molecule deprotonates the alcohol group formed in the previous step, regenerating the carbonyl group.
Comment: It is possible that these two protonation events may occur in a stepwise, rather than concerted, manner.
Amino acids involved in the reaction step.
There are no amino acid residues associated with this reaction step
Metal Cofactors involved in Step 03
View similar reactions in MACiE.