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Entry M0194    5.4.2.8    phosphomannomutase/phosphoglucomutase

Previous Step

Step 02

The 1,6-biphosphate rotates in the active site before Ser108 attacks the 6 phosphate in a nucleophilic substitution reaction, resulting in regeneration of the phosphorylated serine and the D-mannose 1-phosphate product.

GIF of Reaction Step M0194.stg02


Comment: The intermediate must reorient in the active site so that the phosphoryl group brought in by the substrate can be transferred to the enzyme to yield the product and regenerate phopshorylated enzyme that is competent for another round of catalysis [1].



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Formation
Enzyme-Substrate Bond Formation
Bond Cleavage
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His329 Side Chain spectator Electrostatic Stabiliser
Polar Interaction
Arg247 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His109 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Sep108 Post-Translationally Modified Residue reactant Hydrogen Bond Acceptor
Nucleophile
Lys118 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg20 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium ZN 500 x spectator Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
P-O
None
O
P

View similar reactions in MACiE.


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