spacer

Overview for MACiE Entry M0188

Version history

General Information

EC Number: 5.1.3.2 (A member of the Isomerases, Racemases and epimerases, Acting on carbohydrates and derivatives)

Enzyme Name: UDP-glucose 4-epimerase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P09147 - UDP-glucose 4-epimerase

Representative PDB Code: 1xel - UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI (Resolution = 1.80 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of UDP-galactose

right arrow

Image of UDP-glucose

UDP-galactose
C00052
CHEBI:58439
UDP-glucose
C00029
CHEBI:58367

Overall Comment: The rate-determining step of this reaction is the conformational change induced upon binding of the substrate. This is due to the fact that the mutation Tyr149Phe decreases the rate of the hydride transfer and the rate-determining conformational change by the same degree.


View similar reactions


Stepwise Description of the Reaction

Step 1Tyr149 deprotonates the 4-hydroxide of the substrate, resulting in an elimination of the hydride from the C4 position. The hydride then attacks the NAD cofactor in a nucleophilic addition reaction.
Step 2The intermediate undergoes a conformational change before the NAD initiates an elimination of the hydride back to the intermediate, which in turn deprotonates Tyr149.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Ser 124 A Side Chain
Tyr 149 A Side Chain
Lys 153 A Side Chain

Organic Cofactors for M0188

Type Identity Chain
NAD NAD 340 Overview

References

  1. E. Berger et al. (2001), Biochemistry, 40, 6699-6705. Acid-base catalysis by UDP-galactose 4-epimerase: correlations of kinetically measured acid dissociation constants with thermodynamic values for tyrosine 149.
    Medline: 11380265

Homologue information for M0188 (1xel)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0255 alcohol dehydrogenase
1.1.1.1
1mg5 3.40.50.720
0.53331Compare
M0092 UDP-glucose 6-dehydrogenase
1.1.1.22
1dli 3.40.50.720
0.52720.1125Compare
M0021 malate dehydrogenase (oxaloacetate-decarboxylating)
1.1.1.38
1do8 3.40.50.720
0.57570.2999Compare
M0227 GDP-L-fucose synthase
1.1.1.271
1e6u 3.40.50.720
3.90.25.10
0.32750.5142Compare
M0110 D-amino-acid oxidase
1.4.3.3
1c0p 3.40.50.720
0.34480.2144Compare
M0237 pteridine reductase
1.5.1.33
2c7v 3.40.50.720
0.55550.2229Compare
M0116 NAD(P)+ transhydrogenase (AB-specific)
1.6.1.2
1hzz 3.40.50.720
0.50.1125Compare
M0142 ferredoxin-NADP+ reductase
1.18.1.2
1e6e 3.40.50.720
0.32250.0182Compare
M0090 adenosylhomocysteinase
3.3.1.1
1b3r 3.40.50.720
0.53570.1525Compare
M0226 UDP-sulfoquinovose synthase
3.13.1.1
1qrr 3.40.50.720
3.90.25.10
0.64580.5888Compare
M0228 dTDP-glucose 4,6-dehydratase
4.2.1.46
1bxk 3.40.50.720
3.90.25.10
0.57890.3549Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.40.50.720

View a comparison of the other reactions in MACiE with the CATH domain 3.90.25.10


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
UDP-glucose 4-epimerase activity (molecular function)
binding (molecular function)
galactose metabolic process (biological process)
metabolic process (biological process)
cellular metabolic process (biological process)
coenzyme binding (molecular function)
spacer
spacer