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Entry M0185    4.2.99.18    DNA-(apurinic or apyrimidinic site) lyase

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Step 07

The released phosphate deprotonates the hydroxyl group, initiating an elimination of the covalently bound Lys249.

GIF of Reaction Step M0185.stg07



Mechanisms

Bimolecular Elimination

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme-Substrate Bond Cleavage
Bond Order Change
Intermediate Terminated
Intermediate Collapse
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys249 Side Chain reactant Nucleofuge
Asp268 Side Chain spectator Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-N
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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