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Entry M0185    4.2.99.18    DNA-(apurinic or apyrimidinic site) lyase

Next Step

Step 01

Lys249 attacks the DNA 8-oxoguanine substrate in a nucleophilic substitution reaction that yields enzyme bound DNA and the conjugate base of 8-oxoguanine.

GIF of Reaction Step M0185.stg01



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Enzyme-Substrate Bond Formation
Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys249 Side Chain reactant Nucleophile
Asp268 Side Chain spectator Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-N
C-N
None
C
N

View similar reactions in MACiE.


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