spacer

Overview for MACiE Entry M0182

Version history

General Information

EC Number: 4.1.3.30 (A member of the Lyases, Carbon-carbon lyases, Oxo-acid-lyases)

Enzyme Name: methylisocitrate lyase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P77541 - Methylisocitrate lyase

Representative PDB Code: 1mum - STRUCTURE OF THE 2-METHYLISOCITRATE LYASE (PRPB) FROM ESCHERICHIA COLI (Resolution = 1.90 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

right arrow

Image of succinate

Image of pyruvate

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
C04593
CHEBI:57429
succinate
C00042
CHEBI:30031
pyruvate
C00022
CHEBI:15361

View similar reactions


Stepwise Description of the Reaction

Step 1Asp58 deprotonates water which depotonates the alcohol group of the (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate substrate. This initiates an elimination that produces the pyruvate poduct and an intermediate.
Step 2The oxyanion of the intermediate initiates a double bond rearrangement that results in the deprotonatation of Cys123.
Step 3Cys123 deprotonates water and water deprotonates Asp58 to regenerate the active site.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Asp 58 A Side Chain
Cys 123 A Side Chain
Arg 158 A Side Chain
Glu 188 A Side Chain
Asn 210 A Side Chain

Metal Cofactors for M0182

Type Het group Number Chain
magnesium MG 1001 A Overview

References

  1. S. Liu et al. (2005), Biochemistry, 44, 2949-2962. Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution.
    Medline: 15723538

Homologue information for M0182 (1mum)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0326 pyruvate kinase
2.7.1.40
1pkn 3.20.20.60
0.39280.1999Compare
M0207 pyruvate, phosphate dikinase
2.7.9.1
1kc7 3.20.20.60
0.34610.2168Compare
M0272 isocitrate lyase
4.1.3.1
1f61 3.20.20.60
0.16210.1710Compare
M0271 phosphoenolpyruvate mutase
5.4.2.9
1pym 3.20.20.60
0.06250.1922Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.20.20.60


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
catalytic activity (molecular function)
metabolic process (biological process)
spacer
spacer