Entry M0181 22.214.171.124 phosphonoacetaldehyde hydrolase
Previous Step Next Step
Lys53 initiates a double bond rearrangement that causes the reduction of the C=C in the alkylated intermediate and deprotonates water. The activated water attacks the phosphate in a nucleophilic substitution that releases Asp12 and the phosphate product.
Comment: Although reference  separates this step into a protonation of the C=C and cleavage by HO- of the aspartate phosphate bond, we are assuming that both species originate from the same water molecule and thus the reactions are concerted.
Amino acids involved in the reaction step.
Metal Cofactors involved in Step 05
||Bonds Changed in Order
||Atom Types Involved
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
View similar reactions in MACiE.