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Entry M0181    3.11.1.1    phosphonoacetaldehyde hydrolase

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Step 02

The oxyanion deprotonates the covalently attached Lys53.

GIF of Reaction Step M0181.stg02



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His56 Side Chain spectator Hydrogen Bond Donor
Ala45 Main Chain Carbonyl spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Met49 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Arg160 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp12 Side Chain spectator Hydrogen Bond Acceptor
Metal Ligand
Lys53 Side Chain reactant Covalently Attached
Hydrogen Bond Donor
Proton Donor

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium MG 502 x spectator Substrate Binding
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
N-H
None
H
N
O

View similar reactions in MACiE.


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