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Overview for MACiE Entry M0178

Version history

General Information

EC Number: 3.6.3.14 (A member of the Hydrolases, Acting on acid anhydrides, Acting on acid anhydrides to catalyse transmembrane movement of substances)

Enzyme Name: H+-transporting two-sector ATPase

Biological Species: Bos taurus (Cow)

Catalytic Chain UniprotKB Accession Codes:

  • P19483 - ATP synthase subunit alpha, mitochondrial
  • P00829 - ATP synthase subunit beta, mitochondrial

Representative PDB Code: 1e79 - BOVINE F1-ATPASE INHIBITED BY DCCD(DICYCLOHEXYLCARBODIIMIDE) (Resolution = 2.4 Å).

Catalytic CATH Codes:

  • 3.40.50.300 - P-loop containing nucleotide triphosphate hydrolases

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of proton

Image of water

Image of ATP

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Image of proton

Image of phosphate

Image of ADP

proton
C00080
CHEBI:24636
water
C00001
CHEBI:15377
ATP
C00002
CHEBI:15422
proton
C00080
CHEBI:24636
phosphate
C00009
CHEBI:18367
ADP
C00008
CHEBI:456216

Overall Comment: The hydrolysis of ATP by F1-ATPase is coupled to the transmembrane proton transfer in F0. Together they form the F1-F0-adenosine triphosphate synthase. There are three active sites working in a cooperative manner.


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Stepwise Description of the Reaction

Step 1The gamma phopshate of ATP deprotonates the first water, which deprotonates a second water, which attacks the gamma phosphate in a nucleophilic addition resulting in a pentavalent phosphate intermediate.
Step 2The pentavalent phosphate collapses, releasing orthophosphate and ADP.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Lys 162 D Side Chain
Glu 188 D Side Chain
Arg 189 D Side Chain
Arg 373 C Side Chain

Metal Cofactors for M0178

Type Het group Number Chain
magnesium MG 601 A Overview

References

  1. M. Dittrich et al. (2004), Biophys. J., 87, 2954-2967. ATP hydrolysis in the betaTP and betaDP catalytic sites of F1-ATPase.
    Medline: 15315950
  2. C. Gibbons et al. (2000), Nat. Struct. Biol., 7, 1055-1061. The structure of the central stalk in bovine F(1)-ATPase at 2.4 A resolution.
    Medline: 11062563

Homologue information for M0178 (1e79)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0212 nitrogenase
1.18.6.1
1n2c 3.40.50.300
0.08040.2423Compare
M0290 adenylate kinase
2.7.4.3
1zio 3.40.50.300
0.23070.6325Compare
M0154 estrone sulfotransferase
2.8.2.4
1hy3 3.40.50.300
0.28570.1125Compare
M0074 dethiobiotin synthase
6.3.3.3
1bs1 3.40.50.300
0.14280.2161Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.40.50.300


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
nucleotide binding (molecular function)
mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) (cellular component)
angiogenesis (biological process)
negative regulation of endothelial cell proliferation (biological process)
protein binding (molecular function)
ATP binding (molecular function)
mitochondrion (cellular component)
mitochondrial proton-transporting ATP synthase complex (cellular component)
mitochondrial matrix (cellular component)
plasma membrane (cellular component)
obsolete ATP catabolic process (biological process)
lipid metabolic process (biological process)
ion transport (biological process)
negative regulation of cell adhesion involved in substrate-bound cell migration (biological process)
hydrogen-exporting ATPase activity, phosphorylative mechanism (molecular function)
embryo development (biological process)
cell surface (cellular component)
ATP synthesis coupled proton transport (biological process)
ATP hydrolysis coupled proton transport (biological process)
proton transport (biological process)
proton-transporting two-sector ATPase complex (cellular component)
hydrolase activity (molecular function)
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances (molecular function)
ATPase activity (molecular function)
nucleoside-triphosphatase activity (molecular function)
proton-transporting two-sector ATPase complex, catalytic domain (cellular component)
MHC class I protein binding (molecular function)
mitochondrial nucleoid (cellular component)
eukaryotic cell surface binding (molecular function)
proton-transporting ATP synthase complex, catalytic core F(1) (cellular component)
ATP metabolic process (biological process)
proton-transporting ATP synthase activity, rotational mechanism (molecular function)
proton-transporting ATPase activity, rotational mechanism (molecular function)
regulation of intracellular pH (biological process)
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