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Entry M0172    3.4.19.-    isoaspartyl dipeptidase

Previous Step

Step 02

The oxyanion initiates an elimination that cleaves the peptide bond. The amine side of the bond then deprotonates Asp285.

GIF of Reaction Step M0172.stg02


Comment: The products depart the active site, and the binuclear metal centre is recharged with hydroxide via a mechanism not yet known [1].



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp285 Side Chain reactant Hydrogen Bond Donor
Proton Donor

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
zinc ZN 800 x spectator Substrate Binding
zinc ZN 801 x spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-N
O-H
The C-O bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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