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Entry M0171    3.4.17.1    carboxypeptidase A

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Step 02

The oxyanion initiates an elimination that cleaves the peptide bond. The amine side of the bond then deprotonates the carboxylic side.

Rate Determining Step

GIF of Reaction Step M0171.stg02



Mechanisms

Unimolecular Elimination by the Conjugate Base
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu270 Side Chain reactant Hydrogen Bond Donor
Hydrogen Bond Acceptor
Proton Acceptor
Proton Donor
Proton Relay
Arg127 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
zinc ZN 1308 A spectator Substrate Binding
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-N
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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