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Overview for MACiE Entry M0170

Version history

General Information

EC Number: 3.4.15.1 (A member of the Hydrolases, Acting on peptide bonds (peptidases), Peptidyl-dipeptidases)

Enzyme Name: peptidyl-dipeptidase A

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

Representative PDB Code: 1o8a - CRYSTAL STRUCTURE OF HUMAN ANGIOTENSIN CONVERTING ENZYME(NATIVE).REMARK (Resolution = 2.0 Å).

Catalytic CATH Codes:

  • Unassigned Domain

Display structure information

Overall Reaction:

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protein
C00017
CHEBI:33711
water
C00001
CHEBI:15377
dipeptide
C00107
CHEBI:46761
protein
X00144

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Stepwise Description of the Reaction

Step 1Glu384 deprotonates the zinc activated water molecule. This activated hydroxide then attacks the carbonyl carbon of the peptide bond in a nucleophilic addition.
Step 2The amine of the peptide bond deprotonates Glu384.
Step 3The oxyanion initiates an elimination which cleaves the peptide bond, resulting in both products.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 353 A Side Chain
Ala 354 A Main Chain Carbonyl
Glu 384 A Side Chain
His 513 A Side Chain
Tyr 523 A Side Chain

Metal Cofactors for M0170

Type Het group Number Chain
zinc ZN 701 A Overview

References

  1. A. G. Tzakos et al. (2003), Protein Eng., 16, 993-1003. Structure-function discrimination of the N- and C- catalytic domains of human angiotensin-converting enzyme: implications for Cl- activation and peptide hydrolysis mechanisms.
    Medline: 14983080
  2. H. M. Kim et al. (2003), FEBS Letters, 538, 65-70. Crystal structure of Drosophila angiotensin I-converting enzyme bound to captopril and lisinopril.
    Medline: 12633854

Homologue information for M0170 (1o8a)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
proteolysis (biological process)
metallopeptidase activity (molecular function)
peptidyl-dipeptidase activity (molecular function)
membrane (cellular component)
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