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Entry M0169    3.4.14.5    dipeptidyl-peptidase IV

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Step 04

His740 deprotonates water, which attacks the carbonyl carbon bound to Ser630 in a nucleophilic addition.

GIF of Reaction Step M0169.stg04



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Bond Formation
Enzyme-Substrate Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp708 Side Chain spectator Hydrogen Bond Acceptor
His740 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Acceptor
Ser630 Side Chain reactant Covalently Attached
Hydrogen Bond Acceptor
Tyr631 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr547 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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