Overview for MACiE Entry M0169

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General Information

EC Number: (A member of the Hydrolases, Acting on peptide bonds (peptidases), Dipeptidyl-peptidases and tripeptidyl-peptidases)

Enzyme Name: dipeptidyl-peptidase IV

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • P27487 - Dipeptidyl peptidase 4 soluble form

Representative PDB Code: 1pfq - CRYSTAL STRUCTURE OF HUMAN APO DIPEPTIDYL PEPTIDASE IV / CD26 (Resolution = 1.90 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

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Image of L-aminoacyl-Lproline


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Stepwise Description of the Reaction

Step 1His740 in a Ser-His-Asp triad deprotonates Ser630.
Step 2Ser730 attacks the carbonyl carbon of the peptide bond in a nucleophilic addition.
Step 3The oxyanion initiates an elimination reaction that cleaves the peptide bond, releasing the new N-terminus of the protein, which protonates from His740.
Step 4His740 deprotonates water, which attacks the carbonyl carbon bound to Ser630 in a nucleophilic addition.
Step 5The oxyanion initiates an elimination that cleaves the acyl bond to Ser630, releasing the L-aminoacyl-Lproline product. Ser630 then deprotonates His740, regenerating the active site.

View similar reactions (composite manual annotation)

Catalytic Residues Involved

Type Number Chain Location of Function
Tyr 547 A Side Chain
Ser 630 A Side Chain
Tyr 631 A Main Chain Amide
Asp 708 A Side Chain
His 740 A Side Chain


  1. J. R. Bjelke et al. (2004), J. Biol. Chem., 279, 34691-34697. Tyrosine 547 constitutes an essential part of the catalytic mechanism of dipeptidyl peptidase IV.
    Medline: 15175333

Homologue information for M0169 (1pfq)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)

Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
M0248 chloride peroxidase
M0218 triacylglycerol lipase
M0005 carboxypeptidase D
M0217 hydroxynitrilase

View a comparison of the other reactions in MACiE with the CATH domain

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

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response to hypoxia (biological process)
protease binding (molecular function)
regulation of T cell mediated immunity (biological process)
aminopeptidase activity (molecular function)
serine-type endopeptidase activity (molecular function)
receptor activity (molecular function)
receptor binding (molecular function)
protein binding (molecular function)
collagen binding (molecular function)
extracellular region (cellular component)
membrane fraction (cellular component)
soluble fraction (cellular component)
endoplasmic reticulum (cellular component)
Golgi apparatus (cellular component)
plasma membrane (cellular component)
proteolysis (biological process)
cell adhesion (biological process)
peptidase activity (molecular function)
serine-type peptidase activity (molecular function)
dipeptidyl-peptidase activity (molecular function)
positive regulation of cell proliferation (biological process)
cell surface (cellular component)
negative regulation of extracellular matrix disassembly (biological process)
membrane (cellular component)
integral component of membrane (cellular component)
apical plasma membrane (cellular component)
lamellipodium (cellular component)
cell junction (cellular component)
endocytic vesicle (cellular component)
lamellipodium membrane (cellular component)
T cell costimulation (biological process)
regulation of cell-cell adhesion mediated by integrin (biological process)
T cell activation (biological process)
peptide binding (molecular function)
protein homodimerization activity (molecular function)
endothelial cell migration (biological process)
membrane raft (cellular component)
intercellular canaliculus (cellular component)
establishment of localization (biological process)
invadopodium membrane (cellular component)