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Entry M0168    3.4.13.19    membrane dipeptidase

Previous Step

Step 03

The oxyanion initiates an elimination that cleaves the peptide bond, releasing the amino acid products, the N-terminus then protonates from Asp288

GIF of Reaction Step M0168.stg03


Comment: Collapse of the tetrahedral intermediate is not explicitly stated in reference [1]. We assume that the final reprotonation event is carried out by Asp288.



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Order Change
Bond Cleavage
Intermediate Collapse
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His152 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp288 Side Chain reactant Hydrogen Bond Donor
Proton Donor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
zinc ZN 401 A spectator Not Active
zinc ZN 402 A spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-N
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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