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Entry M0168    3.4.13.19    membrane dipeptidase

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Step 02

The activated hydroxide attacks the peptide carbonyl in a nucleophilic addition.

GIF of Reaction Step M0168.stg02



Mechanisms

Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His152 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp288 Side Chain spectator Hydrogen Bond Donor

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
zinc ZN 401 A spectator Substrate Binding
Increase Nucleophilicity
Activator
zinc ZN 402 A spectator Substrate Binding
Increase Nucleophilicity
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
None
The C-O bond changes from a double to single bond
C
O

View similar reactions in MACiE.


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