EC Number: 184.108.40.206 (A member of the Hydrolases, Acting on ether bonds, Ether hydrolases)
Enzyme Name: leukotriene-A4 hydrolase
Biological Species: Homo sapiens (Human)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 1hs6 - STRUCTURE OF LEUKOTRIENE A4 HYDROLASE COMPLEXED WITHBESTATIN. (Resolution = 1.95 Å).
Overall Comment: Leukotriene-A4 hydrolase is a bifunctional zinc metalloenzyme which performs epoxide hydrolysis (shown here) and also shows aminopeptidase activity from a common active site. The mechanism shown here is one of two possibilities. The alternative mechanism suggested includes the formation of an ester intermediate. In this scheme the zinc alone activates and opens the epoxide and a carboxylate of Glu271 attacks the substrate at C6 to form an ester intermediate. In a concerted SN2' reaction this ester can then be attacked by a hydroxo group at C12 and a negative charge can move along the conjugated triene system eventually leading to an alkyl-oxygen cleavage. However such cleavages of esters are rare reactions although not unknown .
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Stepwise Description of the Reaction
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Catalytic Residues Involved
Metal Cofactors for M0166
Homologue information for M0166 (1hs6)
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