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Entry M0165    3.1.31.1    micrococcal nuclease

Previous Step

Step 02

Arg87 deprotonates water in an inferred step.

This reaction step has been inferred

GIF of Reaction Step M0165.stg02


Comment: Distance between NH2 of Arg87 and OE1 of Glu43 is 7.74 Angstroms.



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu43 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Arg35 Side Chain spectator Not Active
Arg87 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
calcium CA 1 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
O-H
O-H
None
H
N
O

View similar reactions in MACiE.


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