Overview for MACiE Entry M0165
EC Number: 126.96.36.199 (A member of the Hydrolases, Acting on ester bonds, Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters)
Enzyme Name: micrococcal nuclease
Biological Species: Staphylococcus aureus (Bacteria)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 2sns - STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASEDON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION (Resolution = 1.50 Å).
Catalytic CATH Codes:
- 188.8.131.52 - OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Display structure information
Overall Comment: This enzyme acts in both DNA and RNA.
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Stepwise Description of the Reaction
|Step 1||Glu43 deprotonates the calcium activated water molecule, which then attacks the DNA phosphate bond in a nucleophilic substitution. The 3' DNA hydroxyl protonates from Arg87.|
|Step 2||Arg87 deprotonates water in an inferred step.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0165
- F. A. Cotton et al. (1979), Proc. Natl Acad. Sci. USA, 76, 2551-2555. Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution.
- E. H. Serpersu et al. (1987), Biochemistry, 26, 1289-1300. Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis.
- D. J. Weber et al. (1992), Proteins, 13, 275-287. NMR docking of a substrate into the X-ray structure of staphylococcal nuclease.
Homologue information for M0165 (2sns)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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