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Overview for MACiE Entry M0164

Version history

General Information

EC Number: 3.1.27.5 (A member of the Hydrolases, Acting on ester bonds, Endoribonucleases producing 3'-phosphomonoesters)

Enzyme Name: pancreatic ribonuclease

Biological Species: Bos taurus (Cow)

Catalytic Chain UniprotKB Accession Codes:

  • P61823 - Ribonuclease pancreatic

Representative PDB Code: 1ruv - RIBONUCLEASE A-URIDINE VANADATE COMPLEX: HIGH RESOLUTIONRESOLUTION X-RAY STRUCTURE (1.3 A) (Resolution = 1.25 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of RNA

Image of water

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Image of RNA 3-phosphate

Image of RNA 5-hydroxyl

RNA
C00046
CHEBI:33699
water
C00001
CHEBI:15377
RNA 3-phosphate
X00071
RNA 5-hydroxyl
X00072

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Stepwise Description of the Reaction

Step 1His12 deprotonates the alcohol of the 5' RNA ribose, which initiates an intramolecular nucleophilic substitution on the phosphate bond of the RNA molecule. The leaving alcohol group is reprotonated from His119.
Step 2His119 deprotonates water which attacks the cyclic phosphate intermediate in a nucleophilic substitution. The leaving alcohol group is reprotonated from His12.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 12 A Side Chain
Lys 41 A Side Chain
His 119 A Side Chain
Phe 120 A Main Chain Amide
Asp 121 A Side Chain

References

  1. R. T. Raines (1998), Chem. Rev., 98, 1045-1065. Ribonuclease A.
    Medline: 11848924

Homologue information for M0164 (1ruv)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
nucleic acid binding (molecular function)
endonuclease activity (molecular function)
pancreatic ribonuclease activity (molecular function)
protein binding (molecular function)
extracellular region (cellular component)
hydrolase activity (molecular function)
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