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Overview for MACiE Entry M0158

Version history

General Information

EC Number: 3.1.6.8 (A member of the Hydrolases, Acting on ester bonds, Sulfuric-ester hydrolases)

Enzyme Name: cerebroside-sulfatase

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • P15289 - Arylsulfatase A component C

Representative PDB Code: 1auk - HUMAN ARYLSULFATASE A (Resolution = 2.10 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of water

Image of cerebroside 3-sulfate

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Image of sulfate

Image of cerebroside

water
C00001
CHEBI:15377
cerebroside 3-sulfate
C06125
sulfate
C00059
CHEBI:45696
cerebroside
C02686
CHEBI:23079

Overall Comment: Residue 69 is formylglycine.


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Stepwise Description of the Reaction

Step 1Asp281 deprotonates the post-translationally modified formylglycine, which attacks the sulfate of the cerebroside 3-sulfate substrate in a nucleophilic substitution, releasing the cerebroside product which is reprotonated from His229.
Step 2His125 deprotonates the other alcohol group of the formylglycine, initiating an elimination reaction that produces the sulfate product, which is reprotonated from Asp281.
Step 3His229 deprotonates a water molecule that attacks the carbonyl carbon of the formylglycine in a nucleophilic addition, the oxyanion is reprotonated by His125.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Fgl 69 A Post-translationally modified residue
Lys 123 A Side Chain
Ser 150 A Side Chain
Arg 73 A Side Chain
His 125 A Side Chain
His 229 A Side Chain
Asp 281 A Side Chain
Lys 302 A Side Chain

Metal Cofactors for M0158

Type Het group Number Chain
magnesium MG 44 x Overview

References

  1. R. von Bulow et al. (2001), J. Mol. Biol., 305, 269-277. Crystal structure of an enzyme-substrate complex provides insight into the interaction between human arylsulfatase A and its substrates during catalysis.
    Medline: 11124905

Homologue information for M0158 (1auk)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0044 alkaline phosphatase
3.1.3.1
1alk 3.40.720.10
0.45450.1773Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

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catalytic activity (molecular function)
arylsulfatase activity (molecular function)
cerebroside-sulfatase activity (molecular function)
calcium ion binding (molecular function)
lysosome (cellular component)
metabolic process (biological process)
sulfuric ester hydrolase activity (molecular function)
hydrolase activity (molecular function)
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